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Registros recuperados: 16 | |
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Saenz-romero, Cuauhtemoc; Lamy, Jean-baptiste; Ducousso, Alexis; Musch, Brigitte; Ehrenmann, Francois; Delzon, Sylvain; Cavers, Stephen; Chalupka, Wladyslaw; Dagdas, Said; Hansen, Jon Kehlet; Lee, Steve J.; Liesebach, Mirko; Rau, Hans-martin; Psomas, Achilleas; Schneck, Volker; Steiner, Wilfried; Zimmermann, Niklaus E.; Kremer, Antoine. |
How temperate forests will respond to climate change is uncertain; projections range from severe decline to increased growth. We conducted field tests of sessile oak (Quercus petraea), a widespread keystone European forest tree species, including more than 150,000 trees sourced from 116 geographically diverse populations. The tests were planted on 23 field sites in six European countries, in order to expose them to a wide range of climates, including sites reflecting future warmer and drier climates. By assessing tree height and survival, our objectives were twofold: (1) to identify the source of differential population responses to climate (genetic differentiation due to past divergent climatic selection versus plastic responses to ongoing climate... |
Tipo: Text |
Palavras-chave: Climatic change; Climatic transfer distance; Mixed model; Quercus petraea; Survival; Tree growth. |
Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00358/46967/46882.pdf |
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Gagnaire, Pierre-alexandre; Lamy, Jean-baptiste; Cornette, Florence; Heurtebise, Serge; Degremont, Lionel; Flahauw, Emilie; Boudry, Pierre; Bierne, Nicolas; Lapegue, Sylvie. |
The Pacific cupped oyster is genetically subdivided into two sister taxa, Crassostrea gigas and C. angulata, which are in contact in the north-western Pacific. The nature and origin of their genetic and taxonomic differentiation remains controversial due the lack of known reproductive barriers and the high degree of morphologic similarity. In particular, whether the presence of ecological and/or intrinsic isolating mechanisms contributes to species divergence is unknown. The recent co-introduction of both taxa into Europe offers a unique opportunity to test how genetic differentiation is maintained under new environmental and demographic conditions. We generated a pseudo-chromosome assembly of the Pacific oyster genome using a combination of BAC-end... |
Tipo: Text |
Palavras-chave: Cupped oysters; Genome assembly; Species divergence; Reproductive barriers; Recombination rate. |
Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00455/56622/58337.pdf |
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Degremont, Lionel; Maurouard, Elise; Godfrin, Yoann; Travers, Marie-agnes; Morga, Benjamin; Lamy, Jean-baptiste; Benabdelmouna, Abdellah. |
Depuis 2012, le nombre de cas de mortalités d’huîtres creuses adultes (Crassostrea gigas) rapportés dans le cadre des réseaux de surveillance de l’Ifremer, et dans lesquels la bactérie Vibrio aestuarianus a été isolée, a fortement augmenté. Les objectifs de cette étude étaient (1) d’étudier la sensibilité des huîtres C. gigas à une infection à V. aestuarianus pour des lots diploïdes et triploïdes et (2) déterminer la réponse à la sélection pour une meilleure résistance à V. aestuarianus pour des huîtres C. gigas. Neuf stocks ont été utilisés, répartis en trois niveaux de sélection, chacun étant donc répliqué par trois pontes. Pour chaque stock, un lot diploïde (2n), un lot triploïde (3n_chim) produit par la rétention d’un globule polaire, et trois lots... |
Tipo: Text |
Palavras-chave: Vibrio aestuarianus; OsHV-1; Sélection; Résistance; Triploïdes; Huître creuse; Crassostrea gigas; Vibrio aestuarianus; OsHV-1; Selective breeding; Disease resistance Pacific oyster; Crassostrea gigas; Triploids. |
Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00404/51530/52140.pdf |
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Marandel, Florianne; Charrier, Gregory; Lamy, Jean-baptiste; Le Cam, Sabrina; Lorance, Pascal; Trenkel, Verena. |
Effective population size (Ne) is a key parameter of population genetics. However, Ne remains challenging to estimate for natural populations as several factors are likely to bias estimates. These factors include sampling design, sequencing method, and data filtering. One issue inherent to the restriction site‐associated DNA sequencing (RADseq) protocol is missing data and SNP selection criteria (e.g., minimum minor allele frequency, number of SNPs). To evaluate the potential impact of SNP selection criteria on Ne estimates (Linkage Disequilibrium method) we used RADseq data for a nonmodel species, the thornback ray. In this data set, the inbreeding coefficient FIS was positively correlated with the amount of missing data, implying data were missing... |
Tipo: Text |
Palavras-chave: Effective population size; Linkage disequilibrium; NeEstimator; RADseq; Skates and rays. |
Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00607/71934/70628.pdf |
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Martenot, Claire; Faury, Nicole; Morga, Benjamin; Degremont, Lionel; Lamy, Jean-baptiste; Houssin, Maryline; Renault, Tristan. |
Viral entry mechanisms of herpesviruses constitute a highly complex process which implicates several viral glycoproteins and different receptors on the host cell surfaces. This initial infection stage was currently undescribed for Ostreid herpes virus 1 (OsHV-1), a herpesvirus infecting bivalves including the Pacific oyster, Crassostrea gigas. To identify OsHV-1 glyproteins implicated in the attachment of the virus to oyster cells, three viral putative membrane proteins, encoded by ORF 25, 41, and 72, were selected and polyclonal antibodies against these targets were used to explore first interactions between the virus and host cells. In addition, effects of dextran sulfate, a negative charged sulfated polysaccharide, were investigated on OsHV-1 infection.... |
Tipo: Text |
Palavras-chave: OsHV-1; Antiviral antibodies; Dextran sulfate; Interaction; Crassostrea gigas; Infection. |
Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00501/61273/64847.pdf |
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Azema, Patrick; Lamy, Jean-baptiste; Boudry, Pierre; Renault, Tristan; Travers, Marie-agnes; Degremont, Lionel. |
Background In France, two main diseases threaten Pacific oyster production. Since 2008, Crassostrea gigas spat have suffered massive losses due to the ostreid herpesvirus OsHV-1, and since 2012, significant mortalities in commercial-size adults have been related to infection by the bacterium Vibrio aestuarianus. The genetic basis for resistance to V. aestuarianus and OsHV-1 and the nature of the genetic correlation between these two traits were investigated by using 20 half-sib sire families, each containing two full-sib families. For each disease, controlled infectious challenges were conducted using naïve oysters that were 3 to 26 months old. In addition, siblings were tested under field, pond and raceway conditions to determine whether laboratory... |
Tipo: Text |
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Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00372/48350/48518.pdf |
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Francois, Cyrille; Quellec, Stéphane; Collewet, Guylaine; Peyramaure, Paul; Musse, Maja; Lamy, Jean-baptiste; Sauriau, Pierre-guy; Haure, Joel. |
High-throughput phenotyping of shellfish’s traits constitutes a challenge due to the specificities of aquatic animals. Appropriate measuring tools have often to be developed or adapted, in order to continuously and automatically acquire data of biomarkers of interest. IMAGIGAS project aimed to explore a non-destructive and non-invasive approach by using magnetic resonance imaging (MRI) to monitor at high-throughput two biomarkers (body weight and temperature) of the pacific oyster, before and during infectious challenges by Ostreid Herpesvirus type 1 (OsHV-1). Pacific oysters and OsHV-1 suspension and contaminated seawater were produced at Ifremer facilities. The experiments were conducted in 2016-2017 by three staffs (Ifremer LGPMM, Irstea IRM Food,... |
Tipo: Text |
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Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00404/51553/52153.pdf |
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Arzul, Isabelle; Bass, David; Figueras, Antonio; Lamy, Jean-baptiste; Paillard, Christine; Furones, Dolors. |
Since 2016, VIVALDI project, supported by the European Union H2020 programme, has aimed to improve the sustainability and competitiveness of the European shellfish industry, which has been hit by a growing number of mortality cases over the recent years. To this end, tools and strategies to better prevent and mitigate the impact of bivalve diseases have been developed through 6 work packages: - In the 1st work package, the diversity and lifecycles of some bivalve pathogens have been investigated. Environmental-based approaches such as passive sensors or the use of magnetic beads and electrochemical biosensors could be useful for pathogen surveillance and the development of early warning systems. - In the 2nd work package, some key mechanisms involved in... |
Tipo: Text |
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Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00603/71518/69938.pdf |
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Pepin, Jean-francois; Benabdelmouna, Abdellah; Bierne, Nicolas; Costes, Louis; Degremont, Lionel; Garcia, Celine; Guesdon, Stephane; Lamy, Jean-baptiste; Le Moine, Olivier; Morga, Benjamin; Normand, Julien; Robert, Stephane; Saunier, Alice; Soletchnik, Patrick; Travers, Marie-agnes. |
Depuis 2014, des épisodes de mortalité anormale affectent les cheptels mytilicoles tant adultes que juvéniles. Dès l’année 2015, une étude spécifique cofinancée par la DPMA et réalisée par l’Ifremer a été initiée, l’action de recherche MORBLEU (Mortalité des moules Bleues). Les années 2015 et 2016 ont permis de dégager des éléments de connaissance et des pistes relatifs aux facteurs biotiques et abiotiques qui semblent intervenir dans le phénomène de hausse de mortalité des moules depuis 2014. Pour l’année 2017, les trois objectifs principaux étaient de : i) poursuivre la description du phénomène de mortalité ainsi que le contexte environnemental associé en effectuant une nouvelle campagne d’études et d’observations in situ, ii) tester des hypothèses... |
Tipo: Text |
Palavras-chave: Moules; Mytilus edulis; Mortalité; Statut cytogénétique; Anomalies génomiques; Populations; Facteurs environnementaux; Ecologie microbienne. |
Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00503/61503/65337.pdf |
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Pepin, Jean-francois; Benabdelmouna, Abdellah; Bierne, Nicolas; Chasselin, Leo; Degremont, Lionel; Guesdon, Stephane; Lamy, Jean-baptiste; Le Moine, Olivier; Normand, Julien; Robert, Stephane; Soletchnik, Patrick. |
Depuis 2014, des épisodes de mortalité anormale affectent les cheptels mytilicoles tant adultes que juvéniles. Dès l’année 2015, une étude spécifique cofinancée par la DPMA et réalisée par l’Ifremer a été initiée, l’action de recherche MORBLEU (Mortalité des moules Bleues). Les années 2015- 2017 ont permis de dégager des éléments de connaissance et des pistes relatifs aux facteurs biotiques et abiotiques qui semblent intervenir dans le phénomène de hausse de mortalité des moules depuis 2014. Pour l’année 2018, les actions d’observation et de suivis du milieu et des moules ont été fortement réduites et trois axes principaux ont été poursuivis : i) l’étude de la relation entre l’évolution des équilibres microbiens planctoniques et l’apparition de mortalités... |
Tipo: Text |
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Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00588/70056/67999.pdf |
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Degremont, Lionel; Lamy, Jean-baptiste; Pepin, Jean-francois; Travers, Marie-agnes; Renault, Tristan. |
The Pacific oyster, Crassostrea gigas, is the most important commercial oyster species cultivated in the world. Meanwhile, the ostreid herpesvirus 1 (OsHV-1) is one of the major pathogens affecting the Pacific oyster, and numerous mortality outbreaks related to this pathogen are now reported worldwide. To assess the genetic basis of resistance to OsHV-1 infection in spat C. gigas and to facilitate breeding programs for such a trait, if any exist, we compared the mortality of half- and full-sib families using three field methods and a controlled challenge by OsHV-1 in the laboratory. In the field, three methods were tested: (A) one family per bag; (B) one family per small soft mesh bag and all families inside one bag; (C) same as the previous methods but... |
Tipo: Text |
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Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00269/38066/36178.pdf |
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Simon, Alexis; Arbiol, Christine; Nielsen, Einar Eg; Couteau, Jérôme; Sussarellu, Rossana; Burgeot, Thierry; Bernard, Ismaël; Coolen, Joop W. P.; Lamy, Jean-baptiste; Robert, Stephane; Skazina, Maria; Strelkov, Petr; Queiroga, Henrique; Cancio, Ibon; Welch, John J.; Viard, Frédérique; Bierne, Nicolas. |
Human‐mediated transport creates secondary contacts between genetically differentiated lineages, bringing new opportunities for gene exchange. When similar introductions occur in different places, they provide informally replicated experiments for studying hybridisation. We here examined 4,279 Mytilus mussels, sampled in Europe and genotyped with 77 ancestry‐informative markers. We identified a type of introduced mussels, called “dock mussels,” associated with port habitats and displaying a particular genetic signal of admixture between M. edulis and the Mediterranean lineage of M. galloprovincialis. These mussels exhibit similarities in their ancestry compositions, regardless of the local native genetic backgrounds and the distance separating colonised... |
Tipo: Text |
Palavras-chave: Admixture; Bentho-pelagic species; Biological introductions; Clines; Ports; Secondary contact. |
Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00594/70600/68791.pdf |
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Marchand, L.; Nsanganwimana, F.; Lamy, Jean-baptiste; Quintela-sabaris, C.; Gonnelli, C.; Colzi, I.; Fletcher, T.; Oustriere, N.; Kolbas, A.; Kidd, P.; Bordas, F.; Newell, P.; Alvarenga, P.; Deletic, A.; Mench, M.. |
Intra-specific variability of root biomass production (RP) of six rooted macrophytes, i.e. Juncus effusus, Phragmites australis, Schoenoplectus lacustris, Typha latifolia, Phalaris arundinacea, and Iris pseudacorus grown from clones, in response to Cu exposure was investigated. Root biomass production varied widely for all these macrophytes in control conditions (0.08 μM) according to the sampling site. Root biomass production of T. latifolia and I. pseudacorus in the 2.5–25 μM Cu range depended on the sampling location but not on the Cu dose in the growth medium. For P. australis, J. effusus, S. lacustris, and P. arundinacea, an intra-specific variability of RP depending on both the sampling location and the Cu-dose was evidenced. This intra-specific... |
Tipo: Text |
Palavras-chave: Copper tolerance; Phytoremediation; Root biomass; Trace element; Wetland. |
Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00201/31183/29869.pdf |
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Broquard, Coralie; Martinez, Anne-sophie; Maurouard, Elise; Lamy, Jean-baptiste; Dégremont, Lionel. |
Understanding sex determination in the Pacific oyster Crassostrea gigas, a sequential hermaphrodite, can provide prospective on the evolution of sex-determining systems for comparative reproduction from an evolutionary perspective. Surprisingly, this mechanism is still poorly understood. To date, sex ratio and sex change have never been studied at the individual level for a large size group and long-term monitoring. To this purpose, we performed an ambitious individual long-term follow-up (6 years) on a large population (cohort 1: 7488 oysters) produced from wild oysters, as well as for a second population produced from the cohort 1 (cohort 2: 4320 oysters). All oysters were individually sexed from 2014 to 2019. For the cohort 1, our results showed a... |
Tipo: Text |
Palavras-chave: Sex-ratio; Sex change; Oysters; Hermaphroditism; Crassostrea gigas. |
Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00587/69868/67845.pdf |
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Azema, Patrick; Maurouard, Elise; Lamy, Jean-baptiste; Degremont, Lionel. |
Mortality associated with ostreid herpesvirus 1 (OsHV-1) has significantly impacted Crassostrea gigas farmers all around the world. Although selective breeding programs to improve OsHV-1 resistance can significantly reduce the mortality rate below 20%, husbandry practices have also been one way to limit or decrease mortality. The main objectives of this study were to describe the influence of two husbandry practices on OsHV-1-induced mortality in C. gigas spat and how different family lines respond to husbandry practices to gain a better understanding of the potential benefits of breeding management as a tool to adapt to improved practices. The first analysis investigated the importance of the size of C. gigas when challenged with OsHV-1 for the first... |
Tipo: Text |
Palavras-chave: Mortality; Crassostrea gigas; Size; OsHV-1; Growing height. |
Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00366/47717/47758.pdf |
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Registros recuperados: 16 | |
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